Hamilton Company专注于精密测量设备，自动化液体处理工作站和样品管理系统的开发，制造和定制。Hamilton的流程针对质量和灵活性进行了优化。无论是具有快速交付时间框架的定制针头，特殊长度的pH传感器，还是全面自动化您的检测工作流程的全面解决方案，相信Hamilton产品将始终满足您的需求。
Hamilton公司60多年来一直是的制造商，总部位于内华达州里诺市; 马萨诸塞州富兰克林; 蒂米什瓦拉，罗马尼亚; 和瑞士的Bonaduz; 和遍布的子公司。
Hamilton为PRP-X100阴离子交换HPLC色谱柱提供了一种快速简便的方法来制备4.0 mM对羟基苯甲酸/ 2.5％甲醇，pH 8.5流动相。
零件号/ REF | 描述 |
79325 | Eluent Concentrate，PRP-X100阴离子交换，（60 mL瓶装，每瓶1瓶） |
79335 | Eluent Concentrate，PRP-X100阴离子交换，（60 mL瓶装，每瓶6瓶） |
Packing Name | Support Material | Exchange Capacity | Pore Size | Temp. Limits | Phase Limits | Buffer Strength | Maximum Pressure |
PRP-1 | PSDVB* | N/A | 100 Å | 5-85 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-C18 | C18 bonded to PSDVB* | N/A | 100 Å | 5-85 °C | pH 1 – 13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-3 | PSDVB* | N/A | 300 Å | 5-85 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-h5 | Fluorinated PSDVB* | N/A | 300 Å | 5-85 °C | pH 1 – 13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
HxSil C18 | Silica | N/A | 110 Å | 5-60 °C | pH 1.5 – 10. 0-100% aqueous, organic modifier | N/A | 6,000 psi |
HxSil C8 | Silica | N/A | 110 Å | 5-60 °C | pH 1.5 – 10. 0-100% aqueous, organic modifier | N/A | 6,000 psi |
Packing Name | Support Material | Exchange Capacity | Pore Size | Temp. Limits | Phase Limits | Buffer Strength | Maximum Pressure |
PRP-X100 | PSDVB* with Tri- methyl ammonium Exchanger | 0.19meq/gm | 100 Å | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-X110 | PSDVB* with Tri-methyl ammonium Exchanger | 0.11meq/gm | 100 Å | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-X500 | Poly(meth-acryl amido-propyl Trimethyl-ammonium chloride) | 1.6meq/gm | Superficially porous | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-2.0N | 5,000 psi |
PRP-X600 | Poly (di-methyl amido-propyl meth-acrylamide) | 1.6meq/gm | Superficially porous | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-1.0N | 5,000 psi |
RCX-10 | PSDVB* with Tri-methyl ammonium Exchanger | 0.35meq/gm | 100 Å | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-1.0N | 5,000 psi |
RCX-30 | PSDVB* with Tri-methyl ammonium Exchanger | 1.0meq/gm | 100 Å | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-1.0N | 5,000 psi |
Packing Name | Support Material | Exchange Capacity | Pore Size | Temp. Limits | Phase Limits | Buffer Strength | Maximum Pressure |
PRP-X200 | PSDVB* Sulfonate Exchanger | 35ueq/gm | 100 Å | 5-60 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-X400 | PSDVB* Sulfonate Exchanger | 2.5meq/gm | N/A | 5-60 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-5.0N | 5,000 psi |
PRP-X800 | PSDVB* Itaconate Exchanger | 1.6meq/gm | 100 Å | 5-60 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
HC-40 Calcium | PSDVB* Sulfonate Exchanger | 5meq/gm | Gel-type | 25-90 °C | 100% Water | Water | 1,000 psi |
HC-75 Calcium | PSDVB* Sulfonate Exchanger | 5meq/gm | Gel-type | 25-90 °C | 100% Water 0-40% Acetonitrile | Water | 400 psi |
HC-75 Hydrogen | PSDVB* Sulfonate Exchanger | 5meq/gm | Gel-type | 25-90 °C | 100% Water 0-40% Acetonitrile | Water | 400 psi |
HC-75 Lead | PSDVB* Sulfonate Exchanger | 5meq/gm | Gel-type | 25-90 °C | 100% Water 0-40% Acetonitrile | Water | 400 psi |
Packing Name | Support Material | Exchange Capacity | Pore Size | Temp. Limits | Phase Limits | Buffer Strength | Maximum Pressure |
PRP-X300 | PSDVB* Sulfonate Exchanger | 0.17meq/gm | 100 Å | 5-60 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
*PSDVB is Poly(styrene-divinylbenzene)
**pH 1-7.9 Temp. 5-60 °C; pH 8-13 Temp. 5-30 °C
HPLC柱具有有限的寿命。建议您使用标准定期监测色谱柱的保留特性和性能。如果您打算长时间储存色谱柱，请使用抑制微生物生长的流动相（含有sodium azide或高浓度甲醇，乙腈等的流动相）。为获得适性能和色谱柱寿命，请遵循以下建议。
恢复色谱柱性能
下面列出的步骤可能会恢复已经污染的色谱柱的性能，但这些过程不会改善损坏或超出其使用寿命的色谱柱的性能。也不能恢复所有固定相。PRP-X500和PRP-X600色谱柱的*支持使得无法通过洗涤程序恢复性能。有关这些色谱柱的技术帮助，请HPLC支持。注意：在执行恢复程序之前，请务必确保流动相是可混溶的，并且不会发生沉淀。如有必要，在转换到新的流动相之前，先用合适的中间溶剂清洗色谱柱。应使用至少五个柱体积的中间溶剂。
色谱柱修复程序
按照下面列出的适当修复程序，使用1.0或2.0 mL / min的流速。除非另有规定。使用每列随附的性能测试报告中列出的测试混合物和条件评估色谱柱性能。
柱修复程序
Packing Material Name
| Restoration Procedure |
PRP-C18 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
PRP-1 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
PRP-3 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
PRP-h5 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
HxSil C8 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
HxSil C18 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
PRP-X100 | Pump approximay 50 mL of methanol with 1% 6 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes. |
PRP-X200 | Inject several times with 100 µL of 1 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes. |
PRP-X300 | Inject several times with 100 µL of 1 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes. |
PRP-X400 | Inject Several times with 100 µL of 0.1 M potassium EDTA. Equilibrate with starting phase for ~ 200 column volumes. |
RCX-10 | Flush with 50 mL 0.1 N sodium hydroxide. Equilibrate with starting phase for ~ 200 column volumes. |
RCX-30 | Flush with 150 mL 0.1 N sodium hydroxide. Equilibrate with starting phase for ~ 200 column volumes. |
HC-40 | Ca2+ Flush with 1% calcium chloride at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes. |
HC-75 | Ca2+ Flush with 1% calcium chloride at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes. |
| H+ Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes. |
| Pb2+ Flush with 1% lead nitrate at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes. |
零件号/ REF | 描述 |
79641 | 分析型HPLC色谱柱，CUSTOM ORDER（1.0,2.1,4.1,4.6 mm ID） |
79669 | 负离子快 |
79670 | 负离子微 |
79668 | 负离子分辨率 |
79643 | 制备型HPLC色谱柱＃1，定制（21.2,30 mm内径） |
79644 | 制备型HPLC色谱柱＃2，定制（50 mm内径） |
79645 | 制备型HPLC色谱柱＃3，CUSTOM ORDER（100 mm ID） |
79421 | PRP-X10010μm2.1x 150 mm |
79346 | PRP-X10010μm2.1x 250 mm |
79439 | PRP-X10010μm4.1x 100 mm |
79434 | PRP-X10010μm4.1x 150 mm |
79433 | PRP-X10010μm4.1x 250 mm |
79365 | PRP-X10010μm4.1x 50 mm |
79354 | PRP-X10010μm4.6x 150 mm PEEK |
79455 | PRP-X10010μm4.6x 250 mm PEEK |
79353 | PRP-X10012-20μm100Å21.2x 250 mm |
79852 | PRP-X1005μm2.1x 150 mm PEEK |
79190 | PRP-X1005μm2.1x 250 mm |
79538 | PRP-X1005μm4.1x 100 mm |
79812 | PRP-X1005μm4.1x 150 mm |
79810 | PRP-X1005μm4.1x 50 mm |
79174 | PRP-X1005μm4.6x 150 mm PEEK |
79181 | PRP-X1005μm4.6x 250 mm PEEK |
79665 | PRP-X1007μm4.6x 150 mm PEEK |
79642 | 半制备型HPLC色谱柱，CUSTOM ORDER（7.0,7.8,10 mm ID） |
货号 | 品名 |
79477 | Analytical Guard Cartridge Holder, PEEK |
32908 | Analytical Guard Cartridge Holder, Stainless Steel |
79293 | Analytical Guard Column, PRP-X110 |
79641 | Analytical HPLC Column, CUSTOM ORDER (1.0, 2.1, 4.1, 4.6 mm ID) |
79643 | Preparative HPLC Column #1, CUSTOM ORDER (21.2, 30 mm ID) |
79644 | Preparative HPLC Column #2, CUSTOM ORDER (50 mm ID) |
79645 | Preparative HPLC Column #3, CUSTOM ORDER (100 mm ID) |
79732 | PRP-X110 7 µm 4.1 x 150 mm |
79734 | PRP-X110 7 µm 4.1 x 250 mm |
79738 | PRP-X110 7 µm 4.6 x 150 mm PEEK |
79729 | PRP-X110 Analytical Guard Column Replacement Cartridges (5/pk), PEEK |
79728 | PRP-X110 Analytical Guard Column Replacement Cartridges (5/pk), Stainless Steel |
79727 | PRP-X110 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), PEEK |
79726 | PRP-X110 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), Stainless Steel |
79827 | PRP-X110, 7 µm Bulk Resin |
79743 | PRP-X110S 7 µm 2.1 x 100 mm PEEK |
79733 | PRP-X110S 7 µm 4.1 x 150 mm |
79735 | PRP-X110S 7 µm 4.1 x 250 mm |
79741 | PRP-X110S 7 µm 4.6 x 250 mm PEEK |
79642 | Semipreparative HPLC Column, CUSTOM ORDER (7.0, 7.8, 10 mm ID) |
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