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The Developmental Studies Hybridoma Bank
39.4D5
| Antigen: Islet-1 & Islet-2 homeobox | Hybridoma Cells Available: Yes | |
| Antigen Species: Rat | Depositor: Jessell, T.M. / Brenner-Morton, S. | |
| Antigen Sequence: | Depositors Institution: HHMI/Columbia University | |
| Antigen Molecular Weight: Predicted: 39kDa; Apparent: 39kDa | Human Protein Atlas: Link | |
| Positive Tested Species Reactivity: altricial fish larva, Chicken, Ferret, Fish, Frog, Human, Mouse, Rat, Zebrafish | Depositors Notes: MN, DRG & other nuclear characterized extensively by immunocytochemistry; works in paraformaldehyde-fixed and paraffin-embedded tissue; does not work in methanol or acetone (see Sanders et al., 2003, and Georgia et al., 2006) TEST | |
| Gene: Isl1 & Isl2 | Immunogen: Recombinant fusion protein containing aa 178-349 of Isl1 protein (C-terminus) | |
| Alternate Gene Names: ISL1, ISL LIM homeobox 1, ISL2; ISL LIM homeobox 2 | Immunogen Sequence: Partial protein | |
| Isotype: MIgG2b | Alternate Antibody Name: anti-islet1; anti-isl1;anti-islet2; anti-isl2 | |
| Clonality: Monoclonal | Alternate Antigen Name: | |
| Epitope Mapped: Yes | Myeloma Strain: NS1 | |
| Epitope Location or Sequence: aa 178-349 of Isl1 protein | Uniprot ID: P61374 P50480 | |
| Host Species: mouse | Entrez Gene ID: 64444 57233 | |
| Additional Characterization: Link | Antibody Registry ID: AB_2314683 | |
| Additional Information: also detects Islet-2. The protein region used for immunisation lacks the LIM motifs shared with other proteins. | ||
| Recommended Applications: Chromatin Immunoprecipitation, Gel Supershift, Immunofluorescence, Immunohistochemistry, Western Blot | ||
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